Haile Gebrselassie's hopes of appearing at the London Olympics were dashed on Sunday as the two-time Olympic 10,000 metre champion could only finish seventh at the Fanny Blankers-Koen Games in Hengelo.
With the Ethiopian Athletics Federation using this meet in Holland as a qualifier for the Games, the 39-year-old needed to finish among the top two athletes from his country.
But he came in sixth among a strong Ethiopian contingent headed by Tariku Bekele, whose brother Kenenisa, the 2004 and 2008 Olympic champion, is likely to get the wild card providing he can prove his fitness.
Gebrselassie had already failed to earn a spot in the marathon side bound for London this summer.
In the 400m, Santos Luguelin of the Dominican Republic posted the second-fastest time of the year, 44.45 seconds, to edge out Britain's Martyn Rooney.
'Blade Runner' Oscar Pistorius finished fifth in 46.35 secs.
American Ronnie Ash won the 110m hurdles in 13.31 from Britain's Andrew Pozzi.
Phillips Idowu, the 2009 world champion, emerged victorious in the triple jump with a leap of 17.31m while fellow Briton Hannah England came home first in the women's 1500m in 4:04.05.