South Wales Scorpions and North Wales Crusaders each provide four players in Wales' 21-man squad for Saturday's international against France at the Racecourse Ground.
Crusaders' Wrexham-born brothers Owain and Iwan Brown, both former Wales Student internationals, are hoping to make their Test debuts on their home ground.
Wales have had to make wholesale changes from their 2011 Four Nations squad due to injuries, retirements and unavailability and are also unable to call on their Australia-based players, which will pave the way for a number of youngsters to play on the big stage.
Castleford's Daniel Fleming joins experienced club-mate Jacob Emmitt in the squad for the first time while Leeds' Ollie Olds, London Broncos' Michael Channing, Wigan's Rhys Pugsley and Keighley's Craig Moss are other potential debutants.
Wales coach Iestyn Harris said: "We'll have a squad with a mixture of youth and experience. There will be a few debutants who are supported by some old hands.
"This match against France is an essential part of our preparations for the 2013 World Cup which we are jointly hosting here in Wales.
"It will enable us to showcase the Welsh talent that we have in both Super League and those who are coming through the ranks at various levels of the game."
Full Wales squad: Andy Bracek (Barrow), Jacob Emmitt, Daniel Fleming (both Castleford), Ross Divorty (Halifax), Danny Jones, Craig Moss (both Keighley), Ollie Olds, Jack Pring (both Leeds), Michael Channing (London Broncos), Iwan Brown, Owain Brown, Christiaan Roets, Kriss Wilkes (all North Wales Crusaders), Jordan James (Salford), Joe Burke, David James, Steve Parry, Lewis Reece (South Wales Scorpions), Rhys Williams (Warrington), Rhodri Lloyd, Rhys Pugsley (both Wigan).