Holders Leicester Tigers will launch their LV=Cup defence against Aviva Premiership champions Saracens on the weekend of November 9-11.
Last year's beaten finalists Northampton begin the competition, now in its fourth season, with a home game against Harlequins, while Premiership newcomers London Welsh will make their debut against Exeter.
Elsewhere in the opening round, Ospreys will be up against 2010-11 winners Gloucester, Newport Gwent Dragons go to Bath, London Irish meet Sale, Cardiff take on Wasps and Scarlets go to Worcester.
English clubs have been allocated to the pools depending on their finish in last season's Premiership.
Teams in Pool 1 play teams in Pool 4 and teams in Pool 2 play teams in Pool 3, with the top team from each pool qualifying for the semi-finals.
LV= Cup 2012/13 pool draw:
Pool 1: Harlequins, Exeter, Gloucester, Newport Gwent Dragons.
Pool 2: Leicester, Sale, Worcester, Cardiff.
Pool 3: Saracens, London Irish, Wasps, Scarlets.
Pool 4: Northampton, Bath, London Welsh, Ospreys.
LV= Cup 2012/13 fixtures:
Northampton Saints v Harlequins
London Welsh v Exeter Chiefs
Bath Rugby v Newport Gwent Dragons
Ospreys v Gloucester Rugby
London Irish v Sale Sharks
Cardiff Blues v London Wasps
Worcester Warriors v Scarlets
Saracens v Leicester Tigers
Harlequins v Bath Rugby
Exeter v Ospreys
Newport Gwent Dragons v Northampton Saints
Gloucester Rugby v London Welsh
Sale Sharks v Saracens
Leicester Tigers v London Irish
Scarlets v Cardiff Blues
London Wasps v Worcester Warriors
Harlequins v London Welsh
Bath Rugby v Exeter Chiefs
Newport Gwent Dragons v Ospreys
Northampton Saints v Gloucester Rugby
Sale Sharks v Scarlets
Leicester Tigers v London Wasps
Saracens v Cardiff Blues
London Irish v Worcester Warriors
London Welsh v Newport Gwent Dragons
Gloucester Rugby v Bath Rugby
Exeter Chiefs v Northampton Saints
Ospreys v Harlequins
London Wasps v Sale Sharks
Scarlets v Leicester Tigers
Worcester Warriors v Saracens
Cardiff Blues v London Irish
- Semi-finals on March 8-10; Final on March 17 (venue tbc)