Heather Watson's run at the Estoril Open ended in the second round in Portugal as she was beaten 6-3 6-1 by fourth seed Petra Cetkovska.
It has nevertheless been a good week for the 19-year-old from Guernsey, who came through three rounds of qualifying before beating Lucie Hradecka of the Czech Republic in round one.
But Cetkovska, another Czech and the world number 28, proved too strong for Watson, who did at least double her games tally from their only previous meeting in New Haven last year.
The teenager saved three match points to avoid a love set but Cetkovska wasted little time in wrapping up victory on her own serve.
Meanwhile, top seed Roberta Vinci of Italy demolished home wild card Maria Joao Koehler 6-2 6-0 in the second round but the defending champion is out, Anabel Medina Garrigues beaten 3-6 6-3 7-5 by fellow Spaniard Silvia Soler-Espinosa, who saved a match point before claiming her compatriot's scalp.
The second and fifth seeds also fell, with Russia's Maria Kirilenko beaten 1-6 6-1 7-5 by Italian qualifier Karin Knapp while Jie Zheng of China was no match for Galina Voskoboeva, the Kazakh winning 6-4 6-1.
Cetkovska will meet sixth seed Kaia Kanepi in the last eight after the Estonian defeated America's Vania King 6-3 6-4.
There were also two retirements, Australian Jarmila Gajdosova pulling out with an ankle injury after losing the first set 7-2 on a tie-break to seventh seed Nadia Petrova, while a foot problem forced ninth seed Polona Hercog out while she trailed Carla Suarez Navarro 6-4 3-0.